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Fig. 5 | Clinical Epigenetics

Fig. 5

From: BRD4 inhibition alleviates mechanical stress-induced TMJ OA-like pathological changes and attenuates TREM1-mediated inflammatory response

Fig. 5

BRD4 inhibition reduces TREM1 induced by mechanical stress and IL-1β. TREM1 inhibition reduces inflammatory factors induced by IL-1β. a qRT-PCR analysis of Trem1 expression in condylar cartilage at 14 days (n = 6). b Immunohistochemical analysis of TREM1 in condylar cartilage (200×) at 14 days (n = 12). TREM1 expressing chondrocytes are denoted (arrow). The sections were scored according to the intensity of the stain in the condyle (0, negative, no staining; 1, faint yellow, mild staining; 2, clay bank, moderate staining; and 3, brown, intense staining). The chondrocytes were treated with IL-1β (10 ng/mL) for 24 h. The cells were concurrently treated with JQ1 (400 nM) or siBRD4. c qRT-PCR analysis of Trem1 in chondrocytes from samples treated as indicated. d qRT-PCR analysis of Trem1, Il-1β, Tnf-α, and Il-6 in chondrocytes from samples treated as indicated. Error bars indicate the standard deviation (*P < 0.05; **P < 0.01)

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