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Fig. 5 | Clinical Epigenetics

Fig. 5

From: UHRF1 downregulation promotes T follicular helper cell differentiation by increasing BCL6 expression in SLE

Fig. 5

UHRF1 deficiency promotes GC responses induced by NP-KLH after immunization with NP-KLH in WT or UHRF1-cKO mice for 14 days. (a, b) The generation of UHRF1-cKO mice, representative gel of PCR identification and western blot analysis in CD4 + T cells for UHRF1 expression in UHRF1-cKO and WT mice. (c) Flow cytometry analysis of Tfh cell markers with CD4+CXCR5+PD1+. (d) Flow cytometry analysis of GC-B cell markers with B220+Fas+GL7+. (e) Immunofluorescence of GCs from WT and UHRF1-cKO mice, representative images of CD3, B220 and PNA staining of DLNs (bar, 50 µm). (f) Levels of serum specific total IgG, IgG1, IgG2a, IgG2b and IgM for NP-KLH immunization at days 0, 7 and 14 by ELISA. All data are shown as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 relative to controls

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