Fig. 4

GSK3β promotes HDAC4 phosphorylation and degradation. a GSK3β mRNA expression. b GSK3β protein expression determined by Western blotting; *p < 0.05 versus control. c GSK3β silencing efficiency determined by RT-qPCR; *p < 0.05 versus sh-NC. d Interaction between GSK3β and HDAC4 determined by Co-IP. e HDAC4 phosphorylation level determined by Western blotting after GSK3β knockdown or overexpression. f HDAC4 and GSK3β protein expression determined by Western blotting after GSK3β knockdown, overexpression, and/or proteasome inhibitor MG132 treatment; *p < 0.05 versus sh-NC; #p < 0.05 versus oe-NC. Cell experiments were performed in triplicate