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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Inhibition of HDAC4 by GSK3β leads to downregulation of KLF5 and ASK1 and prevents the progression of intravertebral disc degeneration

Fig. 1

Silencing of HDAC4 relieves IDD. a Disc height index (DHI). b Representative micrographs showing disc tissues after H&E staining (400×). c HDAC4 mRNA expression determined by RT-qPCR. *p < 0.05 versus control. d HDAC4 protein expression determined by Western blotting; *p < 0.05 versus control. e Identification of NP cells. f Silencing efficiency of HDAC4 expression (mRNA) determined by RT-qPCR; *p < 0.05 versus NC. g Silencing efficiency of HDAC4 expression (protein) determined by Western blotting; *p < 0.05 versus NC. h The in vivo silencing efficiency of sh2-HDAC4 determined by Western blotting; i Disc height index (DHI). j Representative micrographs showing disc tissue after H&E staining (400×). k Representative micrographs showing disc tissue after Safranin O fast green staining (400×). l Apoptosis determined by TUNEL assay; m Bcl-2, Bax, and cleaved caspase-3 protein expression determined by Western blotting. n Mechanical pain threshold. o Thermal sensitivity threshold. *p < 0.05 versus control; #p < 0.05 versus sh-NC. n = 9 for animal experiments; cell experiments were performed in triplicate

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Clinical Epigenetics

ISSN: 1868-7083

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