Fig. 4
a Comparison of paired adjacent normal (and) and tumor (Tu) tissue methylation. Lines connect paired tissue samples. Statistical comparison of paired methylation values was carried out by use of the paired t test and showed significant hypermethylation for tumor tissues (paired two-sided t test, p = 6.1 × 10−19). b Box plot comparison of relative TBR1 methylation levels observed in RCC sample tissues (Tu) and independent metastatic tissue samples (Mtx). Note that in case of multiple metastatic tissue samples mean methylation values were presented in the Mtx group. Notches indicate estimated confidence intervals for group medians