Fig. 5From: Methylation silencing of TGF-β receptor type II is involved in malignant transformation of esophageal squamous cell carcinomaTreatment of ESCC cells in culture increases the expression of TGFBR2. a RT-qPCR to detect levels of TGFBR2 mRNA in Het-1A and ESCC cell lines using GAPDH as a control gene. b RT-qPCR and western blot analysis performed on RNA and protein isolated from KYSE-150 cells exposed to increasing concentrations of 5-Aza-2′-deoxycytidine (mean ± SD. P < 0.001). c RT-qPCR and western blot analysis performed on RNA and protein isolated from KYSE-30 cells exposed to increasing concentrations of 5-Aza-2′-deoxycytidine (mean ± SD. P < 0.001)Back to article page