Fig. 1

EZH2 and H3K27me3 expression patterns in melanoma samples prior to anti-PD-1 therapy. EZH2 and H3K27me3 IHC staining of melanoma metastases in 10-fold and 40-fold magnification (from left to right). a H3K27me3 and EZH2 positive invasion front (70% positive staining) of a lymph node metastasis. b H3K27me3 positive invasion front (50% positive staining) and EZH2 negative staining in a lung metastasis. c H3K27me3 (60% positive staining) and EZH2 (40% positive staining) expression in a liver metastasis. d H3K27me3 and EZH2 positive invasion front (5% positive staining) in a brain metastasis with additional cytoplasmatic expression