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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Widespread loss of the silencing epigenetic mark H3K9me3 in astrocytes and neurons along with hippocampal-dependent cognitive impairment in C9orf72 BAC transgenic mice

Fig. 4

H3K9me3 staining at chromocenters is reduced in neurons of the motor cortex in C9BAC mice. a Representative confocal images of immunofluorescence staining for H3K9me3 (red and white) and for NeuN (green) in the motor cortex from control and C9BAC mice (coronal brain section 40 μm). The images represent a maximum projection for the total nuclear volume. Arrowheads indicate selected NeuN-positive cells, and arrows indicate selected NeuN-negative cells. b, d NeuN-positive (b) and NeuN-negative (d) cells selected from a depicted at higher magnifications, showing individual and merged images of H3K9me3 (white and red), NucBlue (white and blue), and NeuN (green). c, e Quantification of the mean nuclear intensity of H3K9me3 staining (a.u.) in NeuN-positive (c) and NeuN-negative (e) cells. In all graphs, bars represent mean ± SEM. *P< 0.05, ***P< 0.001, unpaired Student’s t test (n = 3 mice, at least 25 NeuN-positive and 15 NeuN-negative cells were analyzed per animal)

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