Fig. 2

The clustered distribution of H3K9me1/me2 marks is unaltered in cultured astrocytes from C9BAC mice. a, b Representative confocal images of the immunofluorescence staining of H3K9me1 (a, white and red) and H3K9me2 (b, white and red) in primary cultures of control and C9BAC astrocytes. Nuclei are stained with DAPI (white and green). Single confocal sections are shown. c, d Quantification of the mean nuclear intensity (a.u.) of H3K9me1 (c, upper graph) and H3K9me2 (d, upper graph) staining. Quantification of the mean nuclear intensity of DAPI (a.u.) is also shown (lower graphs). In all graphs, bars represent mean ± SEM. Non-statistical differences (ns), Student’s t test (n = 3 independent experiments, at least 30 cells were analyzed per condition in each experiment)