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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Epigenetic silencing of IGFBPL1 promotes esophageal cancer growth by activating PI3K-AKT signaling

Fig. 1

IGFBPL1 expression and methylation status in esophageal cancer cells. a The expression of IGFBPL1 was detected by semi-quantitative RT-PCR. H2O, negative control; GAPDH, internal control. 5-AZA, 5-AZA-2′-deoxycytidine. “−” indicates the absence of 5-AZA; “+” indicated the presence of 5-AZA. b MSP results of IGFBPL1 in esophageal cancer cell lines. IVD, in vitro-methylated DNA (methylation control); NL, normal lymphocyte DNA (unmethylation control); H2O, double distilled water; U, unmethylated alleles; M, methylated alleles. c Bisulfite sequencing results of IGFBPL1 in KYSE150, KYSE410, KYSE450, and KYSE510 cells. Double-headed arrow indicates MSP PCR product size was 98 bp and bisulfite sequencing focused on a 287 bp region of the CpG island (from − 115 to + 172) around the IGFBPL1 transcription start site. Filled circles indicate methylated CpG sites. Open circles indicate unmethylated CpG sites; TSS: transcription start site

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