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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Male obesity impacts DNA methylation reprogramming in sperm

Fig. 2

Validation of select methylation values obtained on the Illumina HumanMethylation450 BeadChip using an independent quantitative method. a Confirmation of pyrosequencing assay performance whereby methylation input (x axis) was compared to measured methylation (y axis) using defined mixtures of fully methylated and unmethylated DNAs. Data shown are the mean of triplicate measures. Some standard  deviations  were too small to be visible on the graph. b Comparison of DNA methylation measured on the Illumina platform (x axis) versus that measured by pyrosequencing (y axis) for the same CpG sites for n = 30 individuals. The average of duplicate measures is shown ± SD

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