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Table 1 Sperm samples and clinical semen analysis.

From: Sperm DNA methylation epimutation biomarker for paternal offspring autism susceptibility

Study sample Paternal sperm analysis
Sample Age (years) Father age (years) upon collection Father age (years) at pregnancy Collection date of sample Offspring autism case/control Volume (mL) Concentration (mill/mL) Total of spermatozoa (mill) Progressive spermatozoa (%) Non-progressive spermatozoa (%) Immotile (%) Total motile sperm count (time)
(a) Sperm samples and clinical analysis
 IVI 1 42 42 31 7/27/15 Case 2.2 83 182.6 58 11 31 105.91
 IVI 2 44 45 43 7/28/15 Case 1.5 38 57 42 11 47 23.94
 IVI 3 41 41 38 7/29/15 Case 3 68 204 44 11 45 89.76
 IVI 4 38 38 34 7/29/15 Case 3.4 66 224.4 35 11 54 78.54
 IVI 5 37 37 33 7/30/15 Case 1.4 10 14 50 14 36 7
 IVI 6 39 49 40 8/4/15 Case 2.2 23 50.6 31 26 43 15.69
 IVI 7 41 41 31 8/12/15 Case 6 47 282 69 14 17 194.58
 IVI 8 42 42 32 8/14/15 Case 2.5 91 227.5 60 15 25 136.5
 IVI 9 45 45 35 9/9/15 Case 3.5 87 304.5 50 13 37 152.25
 IVI 10 39 31 9/28/15 Case 3 33.3 99.9 44 9 47 43.96
 IVI 11 45 39 12/21/15 Case 4 120 480 50 12 38 240
 IVI 12 35 37 24 9/5/17 Case 5.4 36.3 196.02 43 3 54 84.29
 IVI 13 46 46 35 3/7/16 Case 4.2 17.6 73.92 60 17 23 44.35
 IVI 14 40 40 36 10/11/16 Control 2.8 141.65 396.62 55 10 35 218.14
 IVI 15 41 41 36 10/11/16 Control 1 34.3 34.3 31 18 51 10.63
 IVI 16 46 46 35 10/17/16 Control 2.2 5.5 12.1 29 3 68 3.51
 IVI 17 44 44 33 10/20/16 Control 4.8 10.5 50.4 42 12 46 21.17
 IVI 18 38 38 31 10/21/16 Control 3.2 1.6 5.12 28 13 59 1.43
 IVI 19 36 36 34 11/3/16 Control 6.8 14 95.2 32 3 65 30.46
 IVI 20 41 41 41 3/22/17 Control 2.2 91.8 201.96 49 14 37 98.96
 IVI 21 42 42 32 5/23/17 Control 1.4 42.1 58.94 37 23 40 21.81
 IVI 22 37 37 29 9/6/17 Control 4.2 54 226.8 52 9 39 117.94
 IVI 23 43 43 36 9/6/17 Control 1.8 13 23.4 23 20 57 5.38
 IVI 24 54 54 27 9/15/17 Control 2.5 52 130 48 7 45 62.4
 IVI 25 38 38 34 9/15/17 Control 5.5 1.7 9.35 11 8 81 1.03
 IVI 26 43 43 33 9/22/17 Control 1.5 96 144 35 17 48 50.4
 Mean ± SD  41 ± 3 42 ± 4 34 ± 5 Offspring autism case 3 ± 1 55 ± 33 184 ± 128 49 ± 11 13 ± 5 38 ± 12 94 ± 71
 Mean ± SD 42 ± 5 42 ± 5 34 ± 3 Offspring control 3 ± 2 43 ± 44 107 ± 114 36 ± 13 12 ± 6 52 ± 14 49 ± 63
 Statistical
comparison
NS NS NS (Case vs. Control), not significant (NS) > 0.05 NS NS NS p < 0.01 NS p < 0.01 NS
(b) Blinded test sample set 1 analysis
 BS 1 Identified case Actual case
 BS 2 Identified control Actual control
 BS 3 Identified control Actual control
 BS 4* Identified control Actual Case*
 BS 5 Identified case Actual case
 BS 6 Identified case Actual case
 BS 7 Identified control Actual control
 BS 8 Identified control Actual control
(c) IVI blinded test sample set 2 analysis
 BS 9 Identified case Actual case
 BS 10 Identified case Actual case
 BS 11 Identified case Actual case
 BS 12 Identified case Actual case
 BS 13 Identified control Actual control
 BS 14* Identified control Actual case*
 BS 15 Identified control Actual control
 BS 16 Identified control Actual control
 BS 17 Identified control Actual control
 BS 18 Identified control Actual control
  1. (a) The samples were provided by IVI-RMA, Valencia, Spain, that collected and performed the clinical analysis of semen volume, sperm concentration and analysis, motility and formal progression analysis, and motility analysis. The mean ± SD for case and control samples is provided. A Students t test was used to assess any statistical differences between case and control groups. (b) Blinded test sample set #1 analysis. The analysis was performed to identify the case or control samples and then confirmed once completed after analysis unblinded. (c) Blinded test sample set #2 analysis. IVI-RMA provided an additional ten blinded samples, and following analysis (Identified) was unblinded to assess accuracy (Actual). The false-negative identifications are indicated with an (*) on the labels