Fig. 2

Methylation patterns of β-globin cluster in BM tissues. a Methylation differences in β-thalassemia patients from CON were shown with *p < 0.05, **p < 0.01 and ***p < 0.001 from t-test. b Methylation level relative to CON (the horizontal dashed line) in TFL (black) or TFH (gray) patients are represented by scatter plot for each CpG site with standard error indicated by bars. *p < 0.05, **p < 0.01, ***p < 0.001 indicated the significant difference in TFH and/or TFL from CON. ^###p < 0.001 indicated significant difference between TFH and THL group. c Methylation levels at CpG sites in HBG promoter verified by bisulfite-treated clone sequencing. ● methylated CpGs; ○ unmethylated CpGs. Count, the numbers of the same clone