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Fig. 6 | Clinical Epigenetics

Fig. 6

From: High-fat diet-induced and genetically inherited obesity differentially alters DNA methylation profile in the germline of adult male rats

Fig. 6

Effect of high-fat diet-induced and genetically inherited obesity on TGF-beta signaling pathway in spermatozoa. a, b Hierarchically clustered heatmap of the differentially methylated CpGs of genes associated with TGF-beta pathway. Black rectangle on the heatmap represents the genes validated by pyrosequencing. c, d Pyrosequencing validation of differentially methylated CpGs of genes involved in TGF-beta signaling in spermatozoa of DIO and GIO groups. Data are expressed as mean ± S.E.M, N = 3 samples pooled per group for methylation sequencing and N = 5 per group for pyrosequencing validation. Asterisks indicate significant differences compared with the CD group (*p < 0.05, **p < 0.01, ***p < 0.001). One-way ANOVA, Bonferroni multiple comparisons test: aP < 0.05, bP < 0.01, cP < 0.001 compared with the LEAN group; eP < 0.05, fP < 0.01, gP < 0.001 compared with the CROb group

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