Fig. 2

Effect of diet-induced (DIO) and genetically inherited obesity (GIO) on the transcript levels of DNA methylating enzymatic machinery, cell type-specific 5-methylcytosine (5-mC) density in the testis and global CpG methylation levels in spermatozoa. a, b Represent the transcript levels of DNA methyltransferases in the testis, c, d represent the 5-mC density in different testicular cell populations and e, f represent the percent CpG methylation levels in the spermatozoa of HFD group compared to CD group and WNIN/Ob group compared to CROb and LEAN groups, respectively. Data are expressed as mean ± S.E.M, N = 6 per group. Asterisks indicate significant differences compared with the CD group (*p < 0.05, **p < 0.01, ***p < 0.001); One-way ANOVA, Bonferroni multiple comparisons test: ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 compared with the LEAN group; ^eP < 0.05, ^fP < 0.01, ^gP < 0.001 compared with the CROb group