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Fig. 3 | Clinical Epigenetics

Fig. 3

From: LncRNA STXBP5-AS1 suppresses stem cell-like properties of pancreatic cancer by epigenetically inhibiting neighboring androglobin gene expression

Fig. 3

STXBP5-AS1 suppressed stemness of PC cells. a Sphere formation of PANC-1 and Mia PaCa-2 cells stably transfected with STXBP5-AS1 plasmid (pSin-STXBP5-AS1) or empty vector (pSin-vector). The total sphere numbers in each well were counted and images were taken at 40× magnification. bd The expression levels of stem markers (Sox2, Bmi1, Lin28 and Nanog) in PANC-1 and Mia PaCa-2 cells stably transfected with STXBP5-AS1 plasmid (pSin-STXBP5-AS1) or empty vector (pSin-vector) were assessed by qRT-PCR and western blot. e Tumor incidence of PANC-1 cells stably transfected with STXBP5-AS1 plasmid (pSin-STXBP5-AS1) or empty vector (pSin-vector); cells were injected into the flank of mice with limiting dilutions as indicated. The number of tumors formed in each group was counted after 4 weeks. *P < 0.05; **P < 0.01

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