Table 1 List of primers used in this study
PCR | Primer | Sequence (5′-3′) | Product Size (bp) | PCR cycles | Annealing temperature (°C) |
|---|---|---|---|---|---|
RT-PCR | ZNF471F | GAGATGACGAGTGAGATGAC | 194 | 32 | 55 |
ZNF471R | TGACTTCCCATCTGCTTCTC | ||||
GAPDHF | GGAGTCAACGGATTTGGT | 206 | 23 | 55 | |
GAPDHR | GTGATGGGATTTCCATTGAT | ||||
qPCR | ZNF471F | GAGATGACGAGTGAGATGAC | 194 | 60 | |
ZNF471R | TGACTTCCCATCTGCTTCTC | ||||
GAPDHF | CCAGCAAGAGCACAAGAGGAA | 114 | 60 | ||
GAPDHR | GGTCTACATGGCAACTCAAGG | ||||
MSP | ZNF471m44 | TTTTGTTTTCGTTTTTTTCGTTC | 223 | 60 | |
ZNF471m6 | ACGCGACTAAACCTTCGCG | ||||
ZNF471u44 | GTTTTGTTTTTGTTTTTTTTGTTT | 228 | 58 | ||
ZNF471u6 | AAAAACACAACTAAACCTTCACA | ||||
BGS | ZNF471BGS1 | GGTTTTTTTTATTTTTTTAGTAGTT | 410 | 41 | 60 |
ZNF471BGS4 | TCCCACAACTCACTCCAATAA |