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Fig. 4 | Clinical Epigenetics

Fig. 4

From: The tumor suppressor Zinc finger protein 471 suppresses breast cancer growth and metastasis through inhibiting AKT and Wnt/β-catenin signaling

Fig. 4

ZNF471 induces cell cycle at S phase and induces apoptosis. a The cell cycle distribution was measured by flow cytometry analysis in vector- and ZNF471-expressing MB231 and YCCB1 cells. Left: Representative flow cytometry plots. Right: Data summary. b BrdU-ELISA assay at 24 h in vector- and ZNF471-transfected MB231 and YCCB1 cells. c The percentages of apoptotic cells were evaluated in vector- and ZNF471-transfected MB231 and YCCB1 cells. Left: Representative flow cytometry plots. Right: Quantitative analysis. d Western blot analysis of apoptotic markers (cleaved-caspase 9, cleaved-PARP, Bax, Bcl-2, and p53). All values are presented as the mean ± SD of at least three independent experiments. SD, standard deviation. **p < 0.01; ***p < 0.001

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Clinical Epigenetics

ISSN: 1868-7083

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