Skip to main content
Fig. 2 | Clinical Epigenetics

Fig. 2

From: The tumor suppressor Zinc finger protein 471 suppresses breast cancer growth and metastasis through inhibiting AKT and Wnt/β-catenin signaling

Fig. 2

ZNF471 is downregulated in breast cancer cell lines and tissues due to promoter methylation. a, b Pharmacological demethylation restored the expression of ZNF471 in breast cancer cell lines, with demethylation of the promoter. M, methylated; U, unmethylated. c High-resolution methylation analysis of ZNF471 promoter by BGS in HMEC, MB231 and YCCB1 cells. ZNF471 promoter methylation levels were detected in breast normal tissues (d) and breast cancer tissues (e). f ZNF471 mRNA expression in primary breast tumor tissues (n = 14) and paired adjacent non-cancerous tissues (n = 14) by qPCR. g ZNF471 promoter methylation in breast cancer tissues and normal breast tissues from an online database (https://methhc.mbc.nctu.edu.tw/). BN: breast normal tissues; BC: breast cancer tissues. h An online database was used to analyze the correlation between the expression of ZNF471 and its promoter methylation in breast cancer (https://methhc.mbc.nctu.edu.tw/). Error bars mean standard deviation (SD); values are presented as the mean ± SD of at least three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001)

Back to article page

Clinical Epigenetics

ISSN: 1868-7083

Contact us