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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Morphine leads to global genome changes in H3K27me3 levels via a Polycomb Repressive Complex 2 (PRC2) self-regulatory mechanism in mESCs

Fig. 2

ChIP-Sequencing binding distribution of H3K27me3 after chronic morphine treatment. a ChIP-Sequencing binding distribution of H3K27me3 after morphine treatment. Total number of peaks in control and morphine treated samples and Venn diagram of H3K27me3 BSs. b Representation of H3K27me3 peaks at ± 3 kb around the TSSs in control and morphine treated samples. c Distribution of the H3K27me3 peaks around ± 3 kb from TSS regions. d Pie-chart showing CpG feature distribution of H3K27me3 peaks in CpG island (belonging to promoter or non-promoter region + 1 kb from TSS), shore (< 2 kb), shelf (< 4 kb) and open sea (the rest of the genome) regions. e Heatmap representation of Log2 (FC) values of DBSs in control and treated samples related to promoters CpG island, shore, shelf and open sea regions. f Functional enrichment analysis showing the most indicative biological functions of the specific genes annotated from binding sites (BSs) and differential binding sites (DBSs). Statistical analyses Bonferroni corrected for p < 0.05

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Clinical Epigenetics

ISSN: 1868-7083

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