Fig. 1

DNA hydroxymethylation is depleted in NDMM and remains locally at active chromatin. a Dot plot of 5hmC global quantification by MS in normal plasma cells from healthy donors (n = 5), and of myeloma cells of patients at diagnosis (n = 39) and b at disease stage (ISS I n = 9; ISS II n = 17; ISS III n = 12; NA n = 1). c 5hmC level-based survival analysis with separation of two groups of NDMM (n = 20 and 19, 1 measurement failure) d Distribution of the 5hmC signal at the different ChromHMM chromatin states in multiple myeloma (n = 40, each left-side boxplot) versus normal plasma cells (n = 5, right sides). e Correlation matrix between 5hmC signal (this study) and the histone marks H3K36me3, H3K27ac, H3K4me3, H3K9me3 and H3K27me3 (ChIP-seq data from the Blueprint Consortium) in MM patients. f Average signal and signal matrix at 5hmC peaks of 5hmC (this study), histone marks (Blueprint) and ATAC-seq [33] in MM patients. ISS, International Staging System, NDMM, Newly Diagnosed Multiple Myeloma