Fig. 2
available at the respective time points. p values shown were obtained with t tests and represent the comparison among patients in the early-PN and late-PN groups at day 3, day 5 or day 7. *p value ≤ 0.05, **p value ≤ 0.01, ***p value ≤ 0.001. Tests were not performed to compare early-PN and late-PN on the last PICU day, in view of the variability of the last day (which also was equal to day 3, day 5 or day 7 for some of the patients). Δd0: β value day 0 minus β value day 0, Δd3: β value day 3 minus β value day 0, Δd5: β value day 5 minus β value day 0, ∆d7: β value day 7 minus β value day 0, ΔLd: β value last PICU day minus β value day 0
Evolution of DNA methylation over time in early-PN versus late-PN patients. Detailed time profiles are shown for four representative CpG-sites distributed over the four major identified patterns of changes in methylation status in PICU. Black lines represent the patients in the early-PN group, and grey lines represent patients in the late-PN group (pooled profiles for early-PN and late-PN patients are shown in Fig. 1). Separate graphs are shown for patients in PICU for at least 3 days, at least 5 days, or at least 7 days. Data are presented as mean and standard error. n represents the number of patients with samples