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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Identification of fetal unmodified and 5-hydroxymethylated CG sites in maternal cell-free DNA for non-invasive prenatal testing

Fig. 5

Detection of T21-specific CG-DMRs by qPCR. Relative quantification of individual T21-specific a uCG-DMRs and b 5hmCG-DMRs located on chromosome 21 using qPCR for replicated cfDNA samples of women pregnant with healthy or T21-diagnosed fetuses. The y-axis indicates the threshold cycle values (CT) calculated in qPCR for the regions selected from Table S4 whose genome coordinates are shown above the graphs. Numerical values of CT inversely correlate to the abundance of the DMR region, indicating higher abundance of the region in the cfDNA samples of pregnant females carrying a T21-diagnosed fetus. Only samples which showed CT values above the set threshold (CT values ≤ 34) were included in the graphs. P-values indicated above were calculated using t-test

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