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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Low-dose DNA demethylating therapy induces reprogramming of diverse cancer-related pathways at the single-cell level

Fig. 2

DNA methylation analysis of DAC-treated HCT116 cells at the single-cell level. a Experimental protocol of the single cell cloning of DAC-treated HCT116 cells. Single cells were isolated by the limiting dilution method, and nine clones were obtained. b Changes of DNA methylation levels in DAC-treated bulk cells. Only partial demethylation was induced by DAC treatment. c Changes of DNA methylation levels in DAC-treated clones. Methylation levels were markedly reduced by DAC treatment, and 12.1–28.2% of TSS200CGIs methylated in HCT116 cells were completely demethylated

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Clinical Epigenetics

ISSN: 1868-7083

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