Table 2 Primer sequences, amplicon size, and number of CpGs analyzed by means of pyrosequencing
From: Reduced mitochondrial D-loop methylation levels in sporadic amyotrophic lateral sclerosis
Region | Primer forward | Primer reverse | Sequencing primer | Amplicon size | Number of CpGs |
|---|---|---|---|---|---|
D-loop | Bio-5′TAGGATGAGGTAGGAATTAAAGATAGATA-3′ | 5′ACATCTAATTCCTACTTCAAAATCAT-3′ | 5′CAAATCTATCACCCTATTAA-3′ | 226 bp | 3 |