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Table 3 Pyrosequencing primer details

From: TWIST1 DNA methylation is a cell marker of airway and parenchymal lung fibroblasts that are differentially methylated in asthma

Target

Forward primer

Reverse primer

Sequencing primer

Assay position

TWIST cg10624122

AGTATATAGTGTTGGGGTGGG

*AGTATATAGTGTTGGGGTGGG

AGGTTAGTTTATGGGTTTTTTAG

5

TWIST1 cg14391419

AGTATATAGTGTTGGGGTGGG

*ACCTTACTCCAACCCAAAAA

TTGGGGTGGGGGTAG

1

HLX cg20454002

ATGGAAGTGAGGGATATATAGGAATTTT

*TCTCTCTCCCAACAATTACCAA

AGGGATATATAGGAATTTTT

2

HLX cg22698272

*GGTGTATTTTTTAGGTTTGTAGT

CCCCTTAAACAATACTCTAAAATTTTTCC

ACAAAAACCTCCTTAATAAAATCT

1

HLX cg12479878

TTTTAGGATTGAAGTTTTTAGGGTTGTT

*CTACCCCTTTTCAAAAAAAACCA

GGTGTAGTAATTTTATAATTGGG

1

HLX cg19306970

AGTTTATGTTTGGGTGTTTGGATAT

*CCCTTAAACCTAAATAATAACAACC

TTTGGGTGTTTGGATATA

1

SKAP1 cg03730533

GTAGTAATTTGATAATAAGAAAAGGTTAGT

*ACCTCCCACCCTTCTCTCCC

TTTAGTTAGGTTTTTAGAATTTTTT

1

  1. *Biotinylated