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Fig. 5 | Clinical Epigenetics

Fig. 5

From: IFN-α confers epigenetic regulation of HBV cccDNA minichromosome by modulating GCN5-mediated succinylation of histone H3K79 to clear HBV cccDNA

Fig. 5

IFN-α inhibits the succinylation of histones on HBV cccDNA minichromosome. a, b HepaRG cells were infected with HBV at an MOI of 600 vp/cell and treated with IFN-α for 3 days at the indicated dose. a The acetylation of histones on cccDNA was determined by ChIP-qPCR 8 dpi in HepaRG cells. b The acetylation of histones on cccDNA was determined by ChIP-qPCR 8 dpi in HepG2-NTCP cells. c The acetylation and succinylation of cccDNA were analyzed by ChIP-qPCR 8 dpi in the cells using pan-Kac and pan-Ksucc antibodies. d The acetylation and succinylation of cccDNA were analyzed by ChIP-qPCR 8 dpi in the cells using pan-Kac and pan-Ksucc antibodies. The mean ± SD of at least three experiments is shown. Statistically significant differences are indicated as follows: **P < 0.01; ***P < 0.001

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Clinical Epigenetics

ISSN: 1868-7083

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