Fig. 1

Histone H3K79 succinylation is anchored on the HBV cccDNA minichromosome. a The succinylation of the cccDNA minichromosome was examined by ChIP-qPCR in the liver of HBV-infected human liver-chimeric mice (n = 3). b, c HepaRG and HepG2-NTCP cells were infected with HBV at an MOI of 600 vp/cell. The succinylation of the cccDNA minichromosome was determined by ChIP-qPCR in the cells. The mean ± SD of at least three experiments is shown. d The succinylation of histone H3K79 in cccDNA minichromosome was analyzed by ChIP-qPCR in livers from HBV-infected human liver-chimeric mice (n = 3). e, f HepaRG and HepG2-NTCP cells were infected with HBV at an MOI of 600 vp/cell. Histone H3K79 succinylation of the cccDNA minichromosome was assessed by ChIP-qPCR in the cells. g The levels of histone H3K79 succinylation were examined by Western blot analysis from the livers of human liver-chimeric mice (n = 3) and HBV-infected human liver-chimeric mice (n = 3). The experiments were repeated at least three times