Association between NPPA promoter methylation and hypertension: results from Gusu cohort and replication in an independent sample

Table 4 The associations of NPPA promoter methylation with blood pressure and hypertension in the replication sample

CpG loci	Systolic blood pressure			Diastolic blood pressure			Prevalent hypertension
CpG loci	β (SE)^a	P	q	β (SE)^a	P	q	OR (95%CI)^b	P	q
Single CpG association
CpG1	− 2.22 (0.60)	< 0.001	0.002	− 1.34 (0.34)	< 0.001	< 0.001	0.82 (0.74–0.91)	< 0.001	0.002
CpG2	− 2.00 (1.11)	0.072	0.093	− 1.51 (0.63)	0.016	0.021	1.05 (0.85–1.29)	0.651	0.976
CpG3	− 1.92 (0.81)	0.018	0.048	− 1.35 (0.45)	0.003	0.007	0.95 (0.84–1.09)	0.481	0.976
CpG4	− 0.87 (0.44)	0.049	0.073	− 0.68 (0.25)	0.006	0.010	1.00 (0.92–1.08)	0.936	0.998
CpG5	− 1.22 (0.57)	0.033	0.059	− 0.89 (0.32)	0.006	0.010	1.00 (0.90–1.11)	0.998	0.998
CpG6	− 1.13 (0.49)	0.021	0.048	− 0.95 (0.28)	< 0.001	0.002	0.94 (0.86–1.02)	0.158	0.475
CpG7	− 1.20 (0.45)	0.007	0.033	− 0.91 (0.25)	< 0.001	0.001	1.02 (0.95–1.11)	0.559	0.976
CpG8	− 0.59 (0.47)	0.203	0.228	− 0.54 (0.22)	0.040	0.045	1.01 (0.93–1.10)	0.780	0.998
CpG9	− 0.05 (0.39)	0.894	0.894	− 0.31 (0.22)	0.160	0.160	1.08 (1.01–1.16)	0.018	0.081
Gene-based association
Average	− 1.29 (0.60)	0.031	-	− 1.04 (0.34)	0.002	-	1.05 (0.94–1.18)	0.360	-
wTPM		< 0.001			< 0.001			0.003

^aβ indicated the change of blood pressure in mmHg associated with per 5% increase in DNA methylation level, adjusting for age, sex, education level, cigarette smoking, alcohol consumption, body mass index, fasting glucose, low- and high-density lipoprotein cholesterol, and antihypertensive medications
^bOdds of having hypertension associated with per 5% increase in DNA methylation level, adjusting for age, sex, education level, cigarette smoking, alcohol consumption, body mass index, fasting glucose, and low- and high-density lipoprotein cholesterol
The values in bold indicate a statistical significance level at < 0.05

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