Table 2 Primer sequences and PCR reaction conditions for EVL/MIR342
From: Epigenetic silencing of miR-342-3p in B cell lymphoma and its impact on autophagy
|  | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Tm/cycles/MgCl2 | Reference |
|---|---|---|---|---|
(I) Methylation-specific PCR (MSP) | ||||
 EVL/MIR342 | ||||
  M-MSP | AGG GAG CGT ATC GCG TTA C | CGG AAA ACG CTA AAC TTA AAA CTA CG | 55 °C/38x/2 mM | NA |
  U-MSP | GGG AGG GAG TGT ATT GTG TTA T | CTC CAA AAA CAC TAA ACT TAA AAC TAC A | 57 °C/35x/1.5 mM | NA |
 E-cadherin | ||||
  M-MSP | GTG GGC GGG TCG TTA GTT TC | CTC ACA AAT ACT TTA CAA TTC CGA CG | 62 °C/40x/2 mM | NA |
  U-MSP | GGT GGG TGG GTT GTT AGT TTT GT | AAC TCA CAA ATA CTT TAC AAT TCC AAC A | 58 °C/40x/2 mM | NA |
(II) Quantitative real-time reverse transcription-PCR | ||||
 EVL (NM_001330221.1) | GCA GCG ACA ATG AGT GAA CA | TGC TGA TCC TGC AAC TTG AC |  |  |
 DNMT1 | GCT ACC TGG CTA AAG TCA AA | CCA TTC CCA CTC TAC GG | NA | [30] |
 E-cadherin | GGA GGA GAG CGG TGG TCA AA | TGT GCA GCT GGC TCA AGT CAA | NA | [31] |
 GAPDH | ACC ACA GTC CAT GCC ATC ACT | TCC ACC ACC CTG TTG CTG TA | NA | [16] |
(III) Cloning of luciferase reporter constructs with SRBS of miR-342-3p | ||||
 MAP1LC3B | AAG GGC TAG CCC GCC TTT TTG GGT AGA AGT | AGG GTC GAC AGT GAG GAC TTT GGG TGT GG | 55 °C/35x/2 mM | NA |