Fig. 2

Methylation and expression of EVL/MIR342 in NHL cell lines. a, b By qRT-PCR, the lower expression of miR-342-3p was associated with EVL/MIR342 methylation as detected by MSP (a) and quantitative bisulfite pyrosequencing (b). c, d The lower expression of EVL, the host gene of miR-342-3p, was associated with EVL/MIR342 methylation as measured by MSP (c) and quantitative bisulfite pyrosequencing (d). e By plotting the expression of miR-342-3p against its host gene EVL, a trend of concordant expression between miR-342-3p and its host gene EVL was demonstrated. f–h Treatment of SU-DHL-16 cells, completely methylated for EVL/MIR342, with 5-AzadC for 6 days led to EVL/MIR342 promoter demethylation as shown by MSP (f), and re-expression of miR-342-3p (g) and EVL (h) as shown by qRT-PCR. qRT-PCR data of 5-AzadC treatment were normalized to untreated control as 1. Columns represented mean +/− 1SD from three qRT-PCR experiments in triplicate