Fig. 4

UniNicE-seq of 5-10 μm human frozen tissue sections and genomic features analysis. a Representative IGV screenshot of the normalized read density of the UniNicE-seq libraries of human fetal and adult tissue sections derived from the kidney, liver, and lung. b Representative IGV screenshot of the normalized read density of the UniNicE-seq libraries of fetal and adult lung tissue sections with ChIP-seq chromatin marks of adult tissue for H3K4me3, H3K4me1, and H3K27Ac. c Enrichment of UniNicE-seq peaks with various genomic features. d Correlation analysis of UniNicE-seq peaks of human fetal and adult tissues. e Profile plot of UniNicE-seq open chromatin signals (peak density) of the human tissue samples in the kidney, liver, and lung tissue-specific active enhancers (AE). f Heatmap representing the enrichment of consensus TF-binding motifs identified in each tissue sample near the UniNicE-seq peaks. Both of the TF binding motifs and the samples are organized by the unsupervised k-means clustering method. The p values of e−6 were considered for the cluster analysis