Fig. 2

iPSC-derived neurons recapitulate the transcription profiles of the fusiform gyrus. a Schematic diagrams show the iPS cell differentiation process. b Heatmap shows expression correlation between iPSC-derived neurons and the 4 brain regions. Color scale represents Pearson’s correlation coefficient (r). P values in each cell line were < 0.01. Genes with FPKM > 1 were used to analyze correlation. WT_N: WT neuron, PSEN1_N: PSEN1^mut neuron, PSEN2_N: PSEN2^mut neuron, APOE4_N: APOE^ε4/ε4 neuron. c Venn diagram of DE genes in PSEN2-N/WT-N at cutoff of fold-change > 2 and AD-FUS/normal at both fold-change > 1.5 and P value < 0.05. d Heatmap shows expression correlation between iPSC-derived neurons and 4 brain regions. Color scale represents Pearson’s correlation coefficient (r). P values in each cell line were < 0.01. Genes with FPKM > 1 were used to analyze correlation. Each row represents neurons derived from iPSCs using various differentiation protocols listed in Table S5. e KEGG enrichment comparison of the 1372 common DE genes and the FUS-specific DE genes. The number at the right of each bar is the corresponding −log10 (P value). f Scatter plot of log2 (fold-change) of the 1372 commonly DE genes in c. Representative AD risk factors are labeled