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Table 3 Impact of oocyte vitrification on expression in humans

From: What impact does oocyte vitrification have on epigenetics and gene expression?

References Materials Number of oocytes and embryos Technology of assessment Studied sequences Conclusions
D’Aurora et al. [122] Supernumerary MII, fresh or vitrified 16 fresh, 16 vitrified RTqPCR DCTN3, DCTN1, 2, and 6, PLK1 No significant differences.
Monzo et al. [121] Unfertilized MII (24–78 h post-fertilization), fresh or vitrified 17 fresh, 36 vitrified Microarray RTqPCR validation Global analysis (Affymetrix, HG-U133 Plus2.0)
3 genes (SLC38a2, TXNRD1, GJA1)
Significant differential expression between the non-cryopreserved and vitrified MII oocyte pools (608 genes with 509 down and 99 upregulated). Many genes of the ubiquitination pathway were downregulated.
Chamayou et al. [123] Supernumerary MII, fresh or vitrified 15 fresh, 15 vitrified RTqPCR NAP1L1, TOP1, H1F0H1, SMC, SCC3, RAD21, SMC1A, SMC1B, STAG3, REC8, CLTA, MAPK6, CKS2, DPPA3, OCT4, FOXJ2 Overall decrease in the expression after oocyte vitrification with 63.3% of mRNA content maintained after vitrification.
Di Pietro et al. [124] Supernumerary MII, fresh or vitrified 10 fresh, 15 vitrified RTqPCR HPRT, GAPDH, CYCLOPHILIN, BMP15, GDF9, FIGLA, OCT4, et TAF4B No significant differences.
  1. MII oocyte at metaphase II stage, RT-qPCR quantitative reverse transcription PCR