Table 3 Impact of oocyte vitrification on expression in humans
From: What impact does oocyte vitrification have on epigenetics and gene expression?
References | Materials | Number of oocytes and embryos | Technology of assessment | Studied sequences | Conclusions |
|---|---|---|---|---|---|
D’Aurora et al. [122] | Supernumerary MII, fresh or vitrified | 16 fresh, 16 vitrified | RTqPCR | DCTN3, DCTN1, 2, and 6, PLK1 | No significant differences. |
Monzo et al. [121] | Unfertilized MII (24–78 h post-fertilization), fresh or vitrified | 17 fresh, 36 vitrified | Microarray RTqPCR validation | Global analysis (Affymetrix, HG-U133 Plus2.0) 3 genes (SLC38a2, TXNRD1, GJA1) | Significant differential expression between the non-cryopreserved and vitrified MII oocyte pools (608 genes with 509 down and 99 upregulated). Many genes of the ubiquitination pathway were downregulated. |
Chamayou et al. [123] | Supernumerary MII, fresh or vitrified | 15 fresh, 15 vitrified | RTqPCR | NAP1L1, TOP1, H1F0H1, SMC, SCC3, RAD21, SMC1A, SMC1B, STAG3, REC8, CLTA, MAPK6, CKS2, DPPA3, OCT4, FOXJ2 | Overall decrease in the expression after oocyte vitrification with 63.3% of mRNA content maintained after vitrification. |
Di Pietro et al. [124] | Supernumerary MII, fresh or vitrified | 10 fresh, 15 vitrified | RTqPCR | HPRT, GAPDH, CYCLOPHILIN, BMP15, GDF9, FIGLA, OCT4, et TAF4B | No significant differences. |