Fig. 3From: Circulating unmethylated CHTOP and INS DNA fragments provide evidence of possible islet cell death in youth with obesity and diabetesValidation of linearity and sensitivity of dPCR assay for measurement of differential methylation of the CHTOP-817. Plasmid DNA or islet DNA-spiked sera were subjected to droplet dPCR for differentially methylated CHTOP-817 at varying ratios or concentrations. a Representative 2-D plots from droplet dPCR output of the gating strategy using the cloned unmethylated and methylated CHTOP gene plasmids. b Droplet dPCR quantitation of mixed plasmid dilution of the cloned unmethylated and methylated CHTOP gene plasmids from a representative single experiment (displayed as copies of DNA/microliter). c Droplet dPCR quantitation of unmethylated CHTOP-817 after serial dilution of human islet DNA into serum from a healthy human donor (data shown are mean ± SEM from 3 independent dilution experiments from the same islet donor). d Droplet dPCR quantitation of methylated CHTOP-817 after serial dilution of human islet DNA into serum from a healthy human donor (data shown are mean ± SEM from 3 independent dilution experiments from the same islet donor). The insets in c and d show an expanded view of the dilution curve at lower DNA concentrationsBack to article page