Fig. 4

Performance of the 5mC-5hmC integrated model for PDAC detection. T-SNE plot (a) and heatmap (b) of FPKM from the paired 5mC and 5hmc data, based on the 51 features comprising the twenty-four 5mC biomarkers and the twenty-seven 5hmC biomarkers. Hierarchical clustering was performed across peaks and samples. c Performance of the 5mC-5hmC integrated model in the training set and the validation set. d Boxplot of wd-score deriving from the integrated model for the PDAC samples and the healthy controls. e Boxplot of the wd-scores from the integrated model for PDAC patients with jaundice and those without jaundice. f Boxplot of the wd-scores from the integrated model for the resectable PDAC patients and the unresectable PDAC patients. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 1e−5, Wilcoxon test. PDAC, pancreatic ductal adenocarcinoma; AUC, area under the curve; wd-score, weighted diagnosis score