Fig. 3

Cell-free 5hmC for detection of PDAC. T-SNE plot (a) and heatmap (b) of 5hmC FPKM from the training set and the validation set based on the 15 differentially hydroxymethylated peaks (DhMPs). Hierarchical clustering was performed across peaks and samples. c Performance of the 5hmC model in the training set and the validation set. d Performance of the 5hmC model in the test data set. e Boxplot of the wd-scores calculating with 5hmC model for PDAC samples and healthy controls. f Boxplot of the wd-scores calculating with 5hmC model for healthy controls, stage I PDAC samples and stage II–IV PDAC samples. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 1e−5, ANOVA. g Genome Browser view of the 5hmC peaks in RABGAP1L gene in chromosome 6 shows a marker locating within the gene (boxed region: chr1: 174799728-174799929). PDAC, pancreatic ductal adenocarcinoma; AUC, area under the curve; wd-score, weighted diagnosis score