Table 1 The sequences of qRT-PCR primers
GeneBank ID | Gene symbol | Primer sequence (forward/reverse) |
|---|---|---|
NM_008084 | Gapdh | 5′-GTGTTCCTACCCCCAATGTGT-3′ 5′-ATTGTCATACCAGGAAATGAGCTT-3′ |
NM_009386 | Tjp1 (ZO-1) | 5′-CCACCTCTGTCCAGCTCTTC-3′ 5′-CACCGGAGTGATGGTTTTCT-3′ |
NM_018778 | Cldn8 | 5′-TCCCAAGGCGTACAGATTTC-3′ 5′-CACTCTCCACTGAGGCATGA-3′ |
NM_134248 | Kim1 (Havcr1) | 5′-AGCTCAGGGTCTCCTTCACA-3′ 5′-ACCACCCCCTTTACTTCCAC-3′ |
NM_008491 | Ngal (Lcn2) | 5′-CTGAATGGGTGGTGAGTGTG-3′ 5′-GGAGTGCTGGCCAAATAAGA-3′ |
NM_031168 | Il-6 | 5′-AGTTGCCTTCTTGGGACTGA-3′ 5′-TCCACGATTTCCCAGAGAAC-3′ |
NM_013693 | Tnf-alpha | 5′-GAAAAGCAAGCAGCCAACCA-3′ 5′-CGGATCATGCTTTCTGTGCTC-3′ |