Fig. 4

Tet2 deficiency boosts inflammation in the kidney upon IR injury. The IR-insulted kidney tissues from WT and Tet2^−/− mice (n = 3 in each genotype) were subjected to RNA-seq analysis. a Scatter plotting of the differentially expressed genes in the IR-insulted kidney tissues from WT and Tet2^−/− mice. Green dot, downregulated genes; red dot, upregulated genes. b Top 10 terms of the pathway analysis of the upregulated DEGs in the IR-insulted kidney between WT and Tet2^−/− mice. c Heatmap showing the augmented expression of renal inflammation-related genes. d, e The mRNA levels of IL6 (d) and TNF-alpha (e) were measured by qRT-PCR. *P < 0.05 and **P < 0.01. N = 3 in each group. f Top 10 terms in the pathway analysis of downregulated DEGs in the IR-insulted kidney between WT and Tet2^−/− mice