Prognostic and predictive value of PD-L2 DNA methylation and mRNA expression in melanoma

Clinical Epigenetics

Table 2 Correlations of PD-L2 methylation and mRNA expression with IFN-γ signature

	IFNG		STAT1		STAT2		STAT3		JAK2		IRF9
Analyte	Spearman’s ρ	P value	Spearman’s ρ	P value	Spearman’s ρ	P value	Spearman’s ρ	P value	Spearman’s ρ	P value	Spearman’s ρ	P value
PD-L2 mRNA	0.81	< 0.001	0.76	< 0.001	0.32	< 0.001	0.36	< 0.001	0.62	< 0.001	0.51	< 0.001
cg14440664	0.16	0.001	0.16	< 0.001	0.21	< 0.001	0.04	0.45	0.14	0.002	0.12	0.008
cg07211259	− 0.46	< 0.001	− 0.46	< 0.001	− 0.34	< 0.001	− 0.13	0.004	− 0.21	< 0.001	− 0.45	< 0.001
cg14351952	0.13	0.004	0.19	< 0.001	0.14	0.002	0.08	0.086	0.23	< 0.001	0.18	< 0.001
cg14133064	− 0.30	< 0.001	− 0.15	0.002	0.03	0.57	− 0.04	0.35	− 0.04	0.42	− 0.13	0.004
cg14374994	0.24	< 0.001	0.21	< 0.001	0.06	0.23	0.12	0.009	0.27	< 0.001	0.12	0.007

Correlations of PD-L2 methylation and mRNA expression with mRNA expression of IFNG and IFN-γ–regulated genes (STAT1, STAT2, STAT3, JAK2, and IRF9). DNA methylation was determined at five different CpG sites, each gene targeted by HumanMethylation450 BeadChip beads (Fig. 1). Significant features are shown in boldface. Data were procurable from N = 468 tumor samples

ISSN: 1868-7083