Fig. 7

Analysis of circulating cell-free tumour DNA using multiplex PCR bisulphite sequencing. a Gel showing successful MBPS libraries (~ 250 bp) of 24 breast cancer-derived circulating cfDNA samples (pre-PCR cleanup). L = ladder. b Boxplot showing full coverage (top panel) across the 33 amplicons of the breast cancer panels from a sequencing run on the 24 cfDNA tumour samples. Bottom panel shows the same data with a different y-axis scale to better show the difference between the lower coverage amplicons, with the dashed line indicating the cut-off (100 reads). c Boxplot of the methylation values detected using the MBPS assay across 24 cfDNA samples