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Fig. 6 | Clinical Epigenetics

Fig. 6

From: Comprehensive evaluation of targeted multiplex bisulphite PCR sequencing for validation of DNA methylation biomarker panels

Fig. 6

Multiplex bisulphite PCR amplicon sequencing of bisulphite-treated FFPET clinical DNA. a TapeStation gel showing 6 representative sequencing libraries from breast cancer FFPET-derived DNA samples are the correct sizes (~ 250 bp). EL = electronic ladder is shown. b Boxplot showing full coverage (top panel) across the 33 amplicons of the breast cancer panels from a sequencing run on our normal and tumour FFPET samples. Bottom panel shows the same data with a different y-axis scale to better show the difference between the lower coverage amplicons, with the dashed line indicating the cut-off (100 reads). c Line plots showing methylation data of 4 representative amplicons across 5 normal and 5 tumour clinical FFPET samples, demonstrating distinct separation between the methylation of normal and tumour samples

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