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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Comprehensive evaluation of targeted multiplex bisulphite PCR sequencing for validation of DNA methylation biomarker panels

Fig. 5

Cross-platform comparison of multiplex bisulphite PCR method and whole-genome bisulphite sequencing methylation data. Barplot shows the difference in methylation between a LNCaP and PrEC and b CAF and NPF for the prostate cancer panels, as measured by WGBS (orange bars) and MBPS (purple bars). The methylation data between the two platforms shows good concordance in determining methylation differences. c, d Representative examples of prostate DMRs corresponding to amplicon 1 (c) and amplicon 32 (d) showing WGBS and multiplex data for each cell line: LNCaP (light pink), PrEC (light green), LNCaP-PrEC (dark blue), CAF (violet), NPF (light brown) and CAF-NPF (light blue). The height of each bar represents the percentage of DNA methylation at each CpG site across the amplicon region

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Clinical Epigenetics

ISSN: 1868-7083

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