Fig. 6

Effects of combined treatment of TICs with UNC0642 and 5-aza-2′-DC sphere forming and cell proliferation capacity of TICs and reactivating G9a target genes in vitro. a Sphere-forming assay on TICs (LCSC4 and LCSC5) following treatment with G9A inhibitor UNC0642 and DNA demethylating agent 5-aza-2′-DC alone and in combination compared to the control (× 10 magnification). b Cell proliferation assay and c qRT PCR of target genes to examine their mRNA level after the cells were treated by UNC0642, 5-aza-2′-DC and with combination of UNC0642 and 5-aza-2′-DC. d QPCR following chromatin immunoprecipitation assays on G9a target genes FOXP1, SP5, and ROBO1. (For t test: *P < 0.05, **P < 0.01, and ***P < 0.001)