Fig. 4

Knockdown of G9A in TICs (LCSC1 and LCSC4) strongly suppressed tumor growth in vivo. a Knockdown of G9A in LCSC1 significantly inhibited the tumor growth in mouse xenografts as shown in growth curves (left panel) and as resected tumors (four replicates for control and five replicates for knockdown, right panel). b Measurement of tumor weight (*P < 0.05 control vs. knocked down) shows the suppression of tumor growth is statistically significant. c Knockdown of G9A in LCSC4 significantly inhibited the tumor growth in mouse xenografts as shown in growth curves (left panel) and as resected tumors (right panel). d Measurement of tumor weight (***P < 0.001 control vs. knocked down) shows the suppression of tumor grown is statistically significant. e IHC staining of the tumors from the mouse xenografts shows reduced G9A expression (for validation of its knockdown) and H3K9Me2 level and increased expression of FOXP1 and SP5 both in LCSC1 and LCSC4 (× 20 magnification). (For t test: *P < 0.05, **P < 0.01, and ***P < 0.001)