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Fig. 2 | Clinical Epigenetics

Fig. 2

From: G9a regulates tumorigenicity and stemness through genome-wide DNA methylation reprogramming in non-small cell lung cancer

Fig. 2

Impact of G9a knockdown on genome-wide methylome and transcriptome changes in patient derived TICs from NSCLC. Genome-wide methylation profiling (850K methylation array) of TICs, i.e., LCSC1 and LCSC4 to examine genes hypermethylated or hypomethylated following G9A knockdown as shown by clustering analysis (red-colored for hypermethylated, green-colored for hypomethylated) (a), G9A knockdown shows that a number of genes are commonly hypomethylated or are hypermethylated between LCSC1 and LCSC4, (b). c RNA sequencing from the TICs (LCSC1 and LCSC4) knocked down with G9A and their controls from LCSC1 and 4 shows that a number of genes are either upregulated (red-colored) or downregulated (green-colored) both in LCSC1 and LCSC4. d A number genes following G9A knockdown were also commonly downregulated or upregulated between LCSC1 and LCSC4. e Of 197 upregulated genes and 67 hypomethylated genes following G9A knockdown, 43 genes were common (whose methylation inversely correlates to their expression) and six genes CDYL2, DPP4, SP5, STAMBPL1, FOXP1, ROBO1 were selected for downstream analyses. f Methylation (β value) obtained from 850K array and g mRNA expression (fold change) level of individual candidate genes obtained from RNA sequencing . (For t test: *P < 0.05, **P < .01, and ***P < 0.001)

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