Fig. 4

Replication of differential methylation at PSORSC13 in this cohort. Top: region around the PSCORSC13 gene, first identified as showing loss of methylation in a separate cohort of depressed subjects who had died by suicide. Symbols are as in Fig. 2 above, with addition of a track for CpG islands (green) often found near promoters. A cluster of probes showing loss of methylation (red, below line) are also evident in our cohort upstream of the first non-coding exon. A pyroassay (pyro, black square) was designed to determine methylation at the individual CpG just 5’ of the island showing greatest loss on the array. Bottom: Table comparing loss of methylation at the indicated CpG site on the array and by pyroassay, which were again in close agreement