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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Effect of short-term prescription opioids on DNA methylation of the OPRM1 promoter

Fig. 4

OPRM1 promoter CpG methylation. a The OPRM1 promoter and the CpG island (green block) are depicted along with the base pair coordinates (black line, GRCh37/hg19) and location of the 10 CpGs (filled circles). Residual β values were extracted after fitting participant ID as a random intercept, and the plots show the methylation patterns across the three visits for CpG1, CpG2, CpG6, and CpG7 (panels with ANOVA line plots; error bars are standard error). The difference between the dosage groups appears at visit 3 (for CpG1, CpG2, CpG6) and visit 2 (for CpG7). The lowest cumulative dose group (< 25 MME: blue dotted line) has lower average methylation compared to the two higher cumulative dose groups (25–90 MME, yellow dashed line; ≥ 90 MME, red solid line). b The promoter mean methylation was taken as the average β values for CpG1 to CpG9. After fitting a regression model with adjustment for age and cell proportions, the leverage plots show a significantly higher average promoter methylation (y-axes) associated with higher MME (x-axis, left panel) and longer duration of use (x-axis, right panel). MME is morphine milligram equivalent

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