BTB/POZ zinc finger protein ZBTB16 inhibits breast cancer proliferation and metastasis through upregulating ZBTB28 and antagonizing BCL6/ZBTB27

Table 3 List of primers used in this study

PCR	Primer	Sequence (5′-3′)	Product size (bp)	PCR cycles	Annealing temperature (°C)
RT-PCR	ZBTB16F	GTCTCCATGGACTTCAGCAC	206 bp	32	55
	ZBTB16R	TACGTCTTCATCCCACTGTG		32
	β-actinF	TCCTGTGGCATCCACGAAACT	315 bp	23	55
	β-actinR	GAAGCATTTGCGGTGGACGAT		23
qRT-PCR	ZBTB16F	GTCTCCATGGACTTCAGCAC	206 bp		60
	ZBTB16R	TACGTCTTCATCCCACTGTG
	VIMF	GACCAGCTAACCAACGACAA	150 bp		60
	VIMR	GTCAACATCCTGTCTGAAAGAT
	CDH2F	CGAATGGATGAAAGACCCATCC	174 bp		60
	CDH2R	GGAGCCACTGCCTTCATAGTCAA
	BCL6F	GACTTCATGTACACATCTCGGC	189 bp		60
	BCL6R	CATCAGCATCCGGCTGTTGA
	ZBTB28F	CTACGTCCGCGAGTTCACTC	170 bp		60
	ZBTB28R	CCCGGAAAATTGAATAGAAG
	β-actinF	GTCTTCCCCTCCATCGTG	113 bp		60
	β-actinR	AGGGTGAGGATGCCTCTCTT
MSP	ZBTB16m3	CGGTGATATCGGAGTTCGTC	139 bp	40	60
	ZBTB16m4	GCGTACGAAAATATACGAAACG		40
	ZBTB16u3	GTGGTGATATTGGAGTTTGTT	142 bp	40	58
	ZBTB16u4	ACACATACAAAAATATACAAAACA		40

ISSN: 1868-7083