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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Single-cell DNA methylation sequencing reveals epigenetic alterations in mouse oocytes superovulated with different dosages of gonadotropins

Fig. 3

Alterations in DNA methylation after superovulation with different dosages of hMG (vs. natural control). a Heatmap of differentially methylated regions (DMRs) (100-CpG window size, corrected p value < 0.05, difference > 20%) between each hMG group and natural control group. Left panel, hMG 5 vs. natural control. Middle panel, hMG 50 vs. natural control. Right panel, hMG 200 vs. natural control. b Pie chart showing the genomic distribution of informative tiles (top) and DMRs (bottom) in each pairwise comparison. c Integrative Genomic Viewer (IGV) screenshot of a 68 kb region showing methylation at the Gfod2 locus, with one tile consistently hypermethylated in the hMG 5 IU, hMG 50 IU, and hMG 200 IU groups. Each vertical bar in the screenshot represents the methylation value (range, 0–100%) of a non-overlapping 100-CpG tile. Genes are shown at the bottom of the screenshot. The treatment of each oocyte is shown on the right of the screenshot. d Boxplot of the methylation levels of the consistently hypermethylated DMRs shown in c

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